WebBackground Information. CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c … WebShop CTCF Rabbit anti-Human, Rat, Canine, Mouse, Primate, Polyclonal, MilliporeSigma™ at Fishersci.com
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WebAnti-CTCF Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS. CoA. References. Technical … WebSonicated chromatin was pre-cleared and incubated with 2 µg of anti-RAD21 antibody (ab992, Abcam), 4 µl anti-CTCF (07729, Millipore), 2 µg anti-PAX5 (sc-1974, Santa Cruz) and 1 µg anti-IgG (sc-2027, Santa Cruz) overnight. The complexes were washed with increasing salt stringency and cross-links were reversed in the presence of 0.2 M NaCl at ...
WebFeb 8, 2008 · The interaction between cohesins and CTCF was functional, since siRNA-mediated knockdown of CTCF abolished cohesin positioning. The preference of CTCF for unmethylated DNA ( Bell and Felsenfeld, 2000, Hark et al., 2000) suggests an explanation for the existence of cell-specific cohesin sites. Our findings provide a mechanism for the … Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, V(D)J recombination and regulation of chromatin architecture.
WebAnti-CTCF Antibody serum, Upstate®; Synonyms: 11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein), … WebAnti-CTCF Antibody serum, Upstate®; Synonyms: 11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein), …
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WebNov 6, 2014 · Sonicated chromatin was pre-cleared and incubated with 2 µg of anti-RAD21 antibody (ab992, Abcam), 4 µl anti-CTCF (07729, Millipore), 2 µg anti-PAX5 (sc-1974, Santa Cruz) and 1 µg anti-IgG (sc-2027, Santa Cruz) overnight. The complexes were washed with increasing salt stringency and cross-links were reversed in the presence of 0.2 M NaCl at ... bircraft ccWebApr 15, 2016 · The role of the zinc finger protein CTCF in organizing the genome within the nucleus is now well established. Widely separated sites on DNA, occupied by both CTCF … bir cover sheetWebAug 17, 2024 · To characterize CTCF expression in vivo, we co-immunostained CTCF with SC-lineage marker SOX10 in sciatic nerves (Fig. 1c).At postnatal day (P) 7, the majority of the SCs marked by SOX10 were also ... dallas tx hotels dealWebNov 3, 2014 · CTCF knockdown in mESCs was performed via lentiviral infection of shRNA as previously described (60, 61). ChIP and Western blot analysis were performed as previously described to confirm CTCF knockdown using antibodies against CTCF (07729; Millipore), normal rabbit IgG (SC2027; Santa Cruz), and α-tubulin (04-1117; Millipore). bircotes historyWebMay 6, 2013 · CTCF has it all. The transcription factor binds to tens of thousands of genomic sites, some tissue-specific, others ultra-conserved. It can act as a transcriptional activator, repressor and insulator, and it can pause transcription. CTCF binds at chromatin domain boundaries, at enhancers and gene pr … birco us armyWebJun 28, 2024 · 4 beds, 2 baths, 1586 sq. ft. multi-family (2-4 unit) located at 5529 NW 7th Ct, Miami, FL 33127 sold for $252,000 on Jun 28, 2024. MLS# A10283083. EXECELLENT … birc reading centerWebOct 6, 2024 · CTCF–CTCF loops and cohesion are both sensitive to RAD21 cleavage, pointing to similarities in the way cohesin associates with DNA as it stabilizes loops at CTCF sites and when it mediates ... bir create account