Invsc1 酵母
Web酿酒酵母菌株 INVSc1 是一种快速生长的二倍体菌株,非常适合表达。INVSc1 具有以下基因型: •INVSc1: MATa his3D1 leu2 trp1-289 ura3-52 MAT his3D1 leu2 trp1-289 ura3-52 Web产品简介:. 本产品用于酿酒酵母蛋白表达实验,可制备诱导培养基平板25块,诱导液体培养基500mL。. 如需获得大量诱导表达产物,诱导培养基可以单独订购。. 套装YM4000包 …
Invsc1 酵母
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WebINVSc1 The genotype and phenotype of the INVSc1 host strain are provided below. Genotype: MATa his3Δ1 leu2 trp1-289 ura3-52/MATα his3Δ1 leu2 trp1-289 ura3-52 Phenotype: His-, Leu-, Trp-, Ura Note that INVSc1 is a diploid strain that is auxotrophic for histidine, leucine, tryptophan, and uracil. The strain will not grow in SC minimal medium ... Web11 apr. 2024 · E.coliNissle1917;USA300:大肠杆菌JM108;希瓦氏菌属MR-1;E.coliW3110野生型;酿酒酵母W303a;酿酒酵母invsc1;酿酒酵母BY4741;酿酒酵母BY4742;毕赤酵母SMD1168H;PseudomonasputidaKT2440;E.coliMC1061;枯草芽孢杆菌WB800;根癌农杆菌EHA105;根癌农杆菌GV3101;CEN.PK2-1D;CEN.PK2-1C; …
WebInvitrogen™ INV Sc 1, S. cerevisiae Yeast Strain Fast-growing diploid strain excellent for expression Manufacturer: Invitrogen™ C81000 Catalog No. C81000 $306.00 / Each of 1 Qty Check Availability Add to cart Description Specifications Customers who viewed this item also viewed Viewing 1 - 3 of 5 Catalog No. 21-377-203 Fisherbrand ™ 96-Well Plates Web12 mrt. 2007 · The experimental procedure involved first sub-cloning the gene encoding my protein of interest into the pYES2/CT vector, then propagating the plasmid in E. coli, transforming yeast INVSc1 with the purified plasmid DNA, expressing the recombinant protein and finally, detecting the expressed protein.
http://www.huayueyang.com.cn/product/277378144 Web菌株简介: INVSC1是酿酒酵母菌株,属于真核细胞。一般的针对原核生物的抗生素例如卡那和氨苄对酵母是无效的,因此为了防止大肠杆菌等原核生物对酵母培养菌株污染,往往 …
WebThe pYES2 vector kit includes 20 µg of expression vector, a TOP10F´ E. coli stab, and an INV S c1 yeast stab. Store the vector at -20°C. Store the TOP10F´ and INV S c1 stabs at …
Web6 mei 2024 · INVSC1是酿酒酵母菌株 ,属于真核细胞。. 一般的针对原核生物的抗生素例如卡那和氨苄对酵母是无效的,因此为了防止大肠杆菌等原核生物对酵母培养菌株污染, … ons household sizeWeb12 apr. 2024 · INVSc1是酿酒酵母,具有氨苄和卡纳抗性。质粒转化条件为电转化,应用于蛋白表达,诱导方法为半乳糖诱导。适宜的生长温度是28-30度,一般使用30度培养,温度超过32度可能导致细胞的死亡。本菌株是生长快速的双倍体酵母细胞,为His,Leu, Trp和Ura营养缺陷型菌株,在缺乏组氨酸,亮氨酸,色氨酸和尿嘧啶的SCminimal media中无法生长。INVSC1酿酒 … ioc in hdfcsecWebinvsc1是酿酒酵母菌株,属于真核细胞。一般的针对原核生物的抗生素例如卡那和氨苄对酵母是无效的,因此为了防止大肠杆菌等原核生物对酵母培养菌株污染,往往会在培养基 … ioc in plasticWeb菌株简介: INVSC1是酿酒酵母菌株,属于真核细胞。一般的针对原核生物的抗生素例如卡那和氨苄对酵母是无效的,因此为了防止大肠杆菌等原核生物对酵母培养菌株污染,往往会在培养基中加入一些氨苄和卡那霉素的抗生素,来抑制细菌菌的污染和生长。酿酒酵母适宜的生长温度是28-30度,一般使用30度培养,温度超过32度可能导致细胞的死亡。INVSC1酿酒 … ioc injury preventionWeb4.将含重组质粒的酿酒酵母菌INVSc1,加入半乳糖诱导重组蛋白CYP27B1的表达,同时设计空载组对照,SDS-PAGE和Western Blot对表达产物进行分析、鉴定。 5.将表达目的蛋白的酿酒酵母表达菌INVSc1于含特定浓度25羟维生素D的诱导培养基中培养24小时,同时设计空载组、酿酒酵母组对照,进行体外转化实验,鉴定其酶活性。 ioc in crowdstrikeWebThe pYES2 vector kit includes 20 µg of expression vector, a TOP10F´ E. coli stab, and an INV S c1 yeast stab. Store the vector at -20°C. Store the TOP10F´ and INV S c1 stabs at +4°C. All reagents are guaranteed stable for 6 months when stored properly. ioc in eyeWebに使用される2倍体出芽酵母株invsc1(インビトロジェン)を用いた。 その結果、nmz004株はもろみを想定した微 酸素環境下(静置培養)で良好な生育を示し、 5%までのグルコース濃度であれば通気培養 (振盪培養)よりも非常に高い増殖を示した。 ioc in networking